lb agar ampicillin plates protocol

Media Preparation: Making agar plates containing

Making agar plates containing Ampicillin X-gal and IPTG You will need to make up the following stocks as well as L-Broth and L-Agar 3% X-gal in DMF: 0 15g in 5 ml DMF (dimethyl formamide) Store at -20 degrees C 100 mM IPGT: 12g in 5ml sterile distilled water Dissolve and filter sterilize Store in aliquots at - 20 degrees C 100 mg/ml

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Preparation of LB liquid medium

Preparation of LB agar plates: (~30-35 plates) Prepare 1L of LB liquid (25g broth powder per 1L) in a 2000mL Erlenmeyer flask Add 15g (1 5%) bacteriological agar Swirl to mix Powder will not dissolve completely that is ok Add a fresh piece of aluminum foil to cover the top and add a fresh piece of autoclave tape

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Microbial Culturing Module

LB ampicillin agar plates are required to select for bacteria that have been transformed with plasmids — like pJET1 2 — which contain the Β-lactamase gene conferring ampicillin resistance to bacteria After transformation bacteria are plated onto LB ampicillin agar plates and incubated at 37C and ampicillin resistant bacterial colonies

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Protocol Making L B Ag ar Pl ates

Protocol To make 500mL of LB agar (makes about 25 LB agar plates): 1 Weigh out the following into a 1L Erlenmeyer flask: 5g NaCl 5g Tryptone 2 5g Yeast Extract 7 5g Agar add dH 2 O to 500mL 2 Note: If your lab has premixed LB agar powder use the suggested amount instead of the other dry ingredients above 3 Swirl to mix the contents do

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How To Make LB Agar Plates

How to make LB agar plates Weigh out 7 5 g agar 5 g tryptone 5 g sodium chloride (NaCl) and 2 5 g yeast extract and add to a 1 L Duran bottle Measure out approximately 400 mL of distilled water and add to the Duran bottle Shake the bottle to dissolve the reagents

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Lb Agar Plate Recipe Ampicillin

Lb agar ampicillin 100 plates pre addgene pouring lb agar plates addgene pouring lb agar plates lb agar plate recipe ampicillin 500mg Lb Agar Ampicillin 100 Plates Pre Poured With μg Addgene Pouring Lb Agar Plates Addgene Pouring Lb Agar Plates Lb Agar Plate Recipe Ampicillin 500mg Lb Agar Plate Recipe Ampicillin 500mg Team Macquarie Protocols Making Lb Agar Plates

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Making LB Agar with ampicillin

Making ampicillin solution Pouring 5cm LB agar plates with ampicillin Pouring 10cm LB agar plates with ampicillin Quality Assurance Avoid contamination of the stock LB agar powder by wearing gloves and using sterile technique when measuring the agar powder Note that ampicillin is temperature-sensitive and can only be added once the agar

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Making LB Agar Plates

The stock ampicillin concentration is 100 mg / mL (i e a 1000X concentration) If you were making up a liter of LB agar to pour onto petri plates you would add 1 mL to 999 mL LB media for a 1000-fold dilution Generally you want to make up less that 1 L because 1 L of media would make about 40 plates (~25 mL each)

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Pouring 5cm LB agar plates with ampicillin

For use by biotechnology students at the ScienceBridge tech sites when pouring 5cm LB agar plates for bacterial culture Objective This SOP sets the safety and procedural specifications for pouring LB agar plates Materials 100 5cm plates 1 LB agar autoclaved with ampicillin 2 5cm petri dishes 3 Autoclaved beaker (for pouring) 4 Red

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Lb Agar Plate Recipe Ampicillin

Lb agar ampicillin 100 plates pre addgene pouring lb agar plates addgene pouring lb agar plates lb agar plate recipe ampicillin 500mg Lb Agar Ampicillin 100 Plates Pre Poured With μg Addgene Pouring Lb Agar Plates Addgene Pouring Lb Agar Plates Lb Agar Plate Recipe Ampicillin 500mg Lb Agar Plate Recipe Ampicillin 500mg Team Macquarie Protocols Making Lb Agar Plates

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A Make Luria broth (LB) and LB agar plates —

A Make Luria broth (LB) and LB agar plates Day 1 LB is a nutrient-rich media that is commonly used to grow bacteria The addition of agar to LB results in the formation of a gel that bacteria can grow on as they are unable to digest the agar but can gather nutrition from the LB within You can also add an antibiotic to the broth The antibiotic allows for the selection of only

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Protocol for pouring LB plates

Protocol for pouring LB plates 1 For 12 plates make a solution of: • 300 mL of DI H 2O • 11 1 g of LB agar powder o To give a concentration of 37 g of LB per 1 L of DI H 2O 2 Microwave the LB media to dissolve the solution 3 Once the solution is fully dissolved place the cap on very loosely or cover flask with aluminum foil 4 Place

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Antibiotic Usage

Follow the recipe card in box for making LB plates being sure to add the agar After autoclaving and when the agar has cooled enough that it's not too hot to touch (about 1 to 1 5hrs) add antibiotics as follows: Ampicillin – add 1ml ampicillin (at 100mg/ml) per liter of agar to obtain a final concentration of 100ug/ml Mark the plate with

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Making agar (+amp) plates

23 02 2015This protocol makes about 40 plates: 30 with ampicillin and 10 without Chemicals LB Nutrient Agar (BIO-RAD #166-0472) 500 mL Demineralized water 2 25 mL Ampicillin (10 mg/mL) Materials Sterile petri dishes Water bath set at 50 degrees Celcius Aluminum foil Cotton Autoclave Protocol Add 13 125 gram of LB Nutrient Agar to 375 mL demineralized water in

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Making LB Agar Plates

The stock ampicillin concentration is 100 mg / mL (i e a 1000X concentration) If you were making up a liter of LB agar to pour onto petri plates you would add 1 mL to 999 mL LB media for a 1000-fold dilution Generally you want to make up less that 1 L because 1 L of media would make about 40 plates (~25 mL each)

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Pouring LB

Pouring LB-Agar+Antibiotic Plates This recipe is for 500 mL of LB agar This makes about 20 10-cm plates (1 bag) 5 g bacto tryptone 2 5 g yeast extract 5 g NaCl 7 5 g bacto agar 500 mL Milli-Q water -OR- Materials you need: • LB Agar Miller (Fisher catalog #: BP1425-500) o 40 grams of LB Agar per 1L of MilliQ water • 2000mL Erlenmeyer flask

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Protocol: Luria

Protocol: Luria-Bertani (LB) Agar Plates with Ampicillin (Amp) Application: Making LB agar plates for bacterial transformation/cloning with Amp selection Procedure: 1 Add 1 L of LB broth into 2 L Erlenmeyer flask 2 Add 15 g Agar 3 Autoclave using liquid cycle 4 Cool to 50 C in H 2O bath 5 Add 0 5 mL Amp stock (100 mg/mL) 6 Pour plates near hot flame for sterilization

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